A New Reversed Phase-HPLC Method Resolving All Major Higher Plant Photosynthetic Pigments
نویسندگان
چکیده
منابع مشابه
Separation and determination of minor photosynthetic pigments by reversed-phase HPLC with minimal alteration of chlorophylls.
Reversed-phase HPLC conditions for separation of chlorophyll (Chl) a, Chl a' (the C132-epimer of Chl a), pheophytin (Pheo) a (the primary electron acceptor of photosystem (PS) II), and phylloquinone (PhQ) (the secondary electron acceptor of PS 1), have been developed. Pigment extraction conditions were optimized in terms of pigment alteration and extraction efficiency. Pigment composition analy...
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Some inorganic anions such as BF4 , CF3COO , ClO4 , and H2PO4 , can affect the solubility of proteins and amino acids. Their effects were observed since 1888 by Fran Hofmeister. During the past decade, these ions have been used increasingly in reversed-phase high-performance liquid chromatography (HPLC), because of their abilities to improve the retention of basic substances in their protonated...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1989
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.91.1.190